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15. | | RODRIGUES, L. A. Z.; ALVES, A. A. C.; PAIVA, L. V.; V, D. Criopreservação de genótipos de mandioca. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 92 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
03/02/2016 |
Data da última atualização: |
01/04/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
TORRES, L. F.; DINIZ, L. E. C.; LIVRAMENTO, K. G. do; FREIRE, L. L.; PAIVA, L. V. |
Afiliação: |
LEANDRO EUGENIO CARDAMONE DINIZ, CPATC. |
Título: |
Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catigua´ MG2 in different cultivation stages. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Acta Physiologiae Plantarum, v. 37, n. 175, p. 2-7, August, 2015. |
DOI: |
10.1007/s11738-015-1924-6 |
Idioma: |
Inglês |
Conteúdo: |
The present study aimed to evaluate the quality of cell suspension of Coffea arabica cv. Catigua´ at four different ages by morphological and gene expression analysis. For this purpose, cell suspension samples were collected at 30, 45, 60 and 75 days. Morphological analysis revealed the presence of embryogenic regions in all samples, and after 60 days, non-embryogenic regions were also identified. The genic analyses revealed that as the cells were transferred to a new medium a change in both their physical and chemical conditions was noticed that caused stress. The decrease in SERK and BBM genes expression after 75th day may also be due to the non-embryogenic regions, characterized by large, elongated and vacuolated cells that were observed in the periphery of embryogenic regions, starting from 60 days of culture. |
Palavras-Chave: |
BBM; Cell suspension; SERK. |
Thesaurus NAL: |
biotechnology; Cell suspension culture; Embryogenesis; somatic embryogenesis; Technology. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01693naa a2200277 a 4500 001 2035999 005 2016-04-01 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1007/s11738-015-1924-6$2DOI 100 1 $aTORRES, L. F. 245 $aGene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catigua´ MG2 in different cultivation stages.$h[electronic resource] 260 $c2015 520 $aThe present study aimed to evaluate the quality of cell suspension of Coffea arabica cv. Catigua´ at four different ages by morphological and gene expression analysis. For this purpose, cell suspension samples were collected at 30, 45, 60 and 75 days. Morphological analysis revealed the presence of embryogenic regions in all samples, and after 60 days, non-embryogenic regions were also identified. The genic analyses revealed that as the cells were transferred to a new medium a change in both their physical and chemical conditions was noticed that caused stress. The decrease in SERK and BBM genes expression after 75th day may also be due to the non-embryogenic regions, characterized by large, elongated and vacuolated cells that were observed in the periphery of embryogenic regions, starting from 60 days of culture. 650 $abiotechnology 650 $aCell suspension culture 650 $aEmbryogenesis 650 $asomatic embryogenesis 650 $aTechnology 653 $aBBM 653 $aCell suspension 653 $aSERK 700 1 $aDINIZ, L. E. C. 700 1 $aLIVRAMENTO, K. G. do 700 1 $aFREIRE, L. L. 700 1 $aPAIVA, L. V. 773 $tActa Physiologiae Plantarum$gv. 37, n. 175, p. 2-7, August, 2015.
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